ENES
Facility

Flow Cytometry & Cell Sorting

Offers expert services for flow cytometry analyses and precise sorting of cell populations. Provides guidance to ensure efficient, reliable, and high-quality experimental results.

Overview

What is Flow Cytometry?

Flow cytometry is a powerful technique used to rapidly measure and analyze multiple physical and chemical characteristics of microscopic particles—such as cells, nuclei, or mitochondria—individually and in suspension. Each particle is evaluated based on parameters like size (forward scatter, FSC), internal complexity or granularity (side scatter, SSC), and fluorescence intensity. During analysis, particles are carried in a fluid stream and pass in single file through one or more laser beams. These lasers excite fluorescent markers that have been attached to the particles, enabling the detection of specific features or molecules. Some flow cytometers, known as cell sorters, are also equipped with specialized modules that allow for the physical separation and collection of specific subpopulations of interest. These sorted cells can be used in downstream functional assays or other experimental applications.

flow capabilities

Service menu

Capabilities are grouped into two practical tiers so users can scan the service scope before requesting support.

A

Core analysis

Routine cytometry readouts for common sample characterization and cell-state measurements.

  1. 01
    Detection of surface and intracellular antigens

    Detección de antígenos de superficie e intracelulares

  2. 02
    Cell counting

    Contaje celular

  3. 03
    Transfection efficiency determination

    Determinación de eficiencia de transfección

  4. 04
    Cell kinetics studies

    Estudios de cinética celular

  5. 05
    Cell cycle analysis

    Análisis del ciclo celular.

  6. 06
    Cell viability, necrosis, and apoptosis analysis

    Análisis de viabilidad celular, necrosis y apoptosis

  7. 07
    Cell proliferation analysis

    Análisis de proliferación celular

B

Advanced assays and sorting

Higher-complexity assays, rare population analysis, nanometric particles and high-speed sorting.

  1. 01
    Soluble cytokine detection assays

    Ensayos de detección de citoquinas solubles

  2. 02
    DNA content analysis

    Análisis de contenido de ADN

  3. 03
    Ploidy determination

    Determinación de ploidía

  4. 04
    Multiparametric analysis of up to 16 colors, functional assays, and gene expression

    Análisis multiparamétricos de hasta 16 colores, funcionales y de expresión génica

  5. 05
    Analysis of complex and/or rare populations

    Análisis de poblaciones complejas y/o poco frecuentes

  6. 06
    Nanometric-scale microparticle analysis (>90 nm)

    Análisis de micropartículas a escala nanométrica (> 90 nm)

  7. 07
    High-speed cell population sorting by cell sorting (enrichment, purity, or single-cell sorting)

    Separación de poblaciones celulares por cell sorting a alta velocidad (enriquecimiento, pureza o single cell)